Recent Faculty Publication: "A novel tetracycline-responsive transgenic mouse strain for skeletal muscle-specific gene expression."

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Skelet Muscle. 2018 Oct 27;8(1):33. doi: 10.1186/s13395-018-0181-y.

A novel tetracycline-responsive transgenic mouse strain for skeletal muscle-specific gene expression.

Abstract

BACKGROUND:

The tetracycline-responsive system (Tet-ON/OFF) has proven to be a valuable tool for manipulating gene expression in an inducible, temporal, and tissue-specific manner. The purpose of this study was to create and characterize a new transgenic mouse strain utilizing the human skeletal muscle α-actin (HSA) promoter to drive skeletal muscle-specific expression of the reverse tetracycline transactivator (rtTA) gene which we have designated as the HSA-rtTA mouse.

METHODS:

To confirm the HSA-rtTA mouse was capable of driving skeletal muscle-specific expression, we crossed the HSA-rtTA mouse with the tetracycline-responsive histone H2B-green fluorescent protein (H2B-GFP) transgenic mouse in order to label myonuclei.

RESULTS:

Reverse transcription-PCR confirmed skeletal muscle-specific expression of rtTA mRNA, while single-fiber analysis showed highly effective GFP labeling of myonuclei in both fast- and slow-twitch skeletal muscles. Pax7 immunohistochemistry of skeletal muscle cross-sections revealed no appreciable GFP expression in satellite cells.

CONCLUSIONS:

The HSA-rtTA transgenic mouse allows for robust, specific, and inducible gene expression across muscles of different fiber types. The HSA-rtTA mouse provides a powerful tool to manipulate gene expression in skeletal muscle.

KEYWORDS:

Skeletal muscle-specific; Tetracycline-responsive

PMID: 30368256
DOI: 10.1186/s13395-018-0181-y