Cata won University-wide 3-Minute Thesis competition. See her winning presentation at: http://youtu.be/OLfU1-W-MpU
- (859) 323-8729 (office)
- (859) 323-8730 (lab)
- 800 Rose Street, Medical Science Building, Rm: MS-615, Lexington, KY 40536-0298
MS, PhD, Moscow Institute of Physics and Technology & Cardiology Research Center, USSR, 1978-1988
Lab Chief, Research Center for Audiology and Hearing Rehabilitation, Moscow, Russia, 1990-1997
Postdoc, National Institute on Deafness and other Communication Disorders, NIH, Bethesda, MD, 1997-2005
Assistant/Associate Professor, Department of Physiology, University of Kentucky, Lexington, KY, 2005-present
Our goal is to understand the mechanisms of mechanosensitivity and active force generation in the sensory cells of the mammalian inner ear, the hair cells. This research is important not only because it is crucial for understanding our senses of hearing and balance, but also because hair cell studies often establish methodologies and serve as a model for exploring molecular mechanisms of mechanotransduction in other systems. Since most cases of severe hearing loss and deafness are associated with the damage to the inner ear hair cells, our experimental models are often relevant to the different types of deafness in humans.
We use mouse models with targeted gene deletion (knockout) or deafness-related mutations to determine the function of specific proteins. The function is assessed at the whole animal, cellular, and molecular levels. Patch-clamp, calcium imaging, live cell confocal microscopy, scanning electron microscopy, and audiology techniques are widely used. We also develop emerging nanotechniques to visualize individual proteins at the surface of a living cell and to probe their function. Prospective post-doctoral fellows and graduate students are expected to learn cutting edge techniques of cell physiology and cell biology and collaborate with a number of laboratories across the country and overseas.
NIDCD/NIH: R01 DC014658, R01 DC012564
Indzhykulian AA, Stepanyan RS, Nelina A, Spinelli KJ, Ahmed ZM, Belyantseva IA, Friedman TB, Barr-Gillespie PG, Frolenkov GI (2013) Molecular Remodeling of Tip Links Underlies Mechanosensory Regeneration in Auditory Hair Cells. PLoS Biol., Jun, 11(6):e1001583 (featured on the cover).
This study in the news: Cell, NIH Research Matters, UKy News, PLoS Biology, NIDCD/NIH, Science Daily.
Riazuddin S, Belyantseva IA, Giese A, Lee K, Indzhykulian AA, Nandamuri SP, Yousaf R, Shinha GP, Lee S, Terrell D, Hegde RS, Ali RA, Anwar S, Andrade-Elizondo PB, Sirmaci A, Parise LV, Basit S, Wali A, Ayub M, Ansar M, Ahmad W, Khan SN, Akram J, Tekin M, Riazuddin Sh, Cook T, Buschbeck EK, Frolenkov GI, Leal SM, Friedman TB, Ahmed ZM. (2012) Mutations of CIB2, a calcium and integrin binding protein, cause Usher syndrome type 1J and nonsyndromic deafness DFNB48. Nat. Genet. 44(11):1265-71.
This study in the news: Science Daily, Daily Mail (UK), US News.
Kitajiri S, Sakamoto T, Belyantseva IA, Goodyear RJ, Stepanyan R, Fujiwara I, Bird JE, Riazuddin S, Riazuddin S, Ahmed ZM, Hinshaw JE, Sellers J, Bartles JR, Hammer JA 3rd, Richardson GP, Griffith AJ, Frolenkov GI, Friedman TB. (2010) Actin-bundling protein TRIOBP forms resilient rootlets of hair cell stereocilia essential for hearing. Cell 141: 786-798 (featured on the cover).
This study in the news: NIDCD/NIH, Cell.
Novak P, Li C, Shevchuk AI, Stepanyan R, Caldwell M, Hughes S, Smart TG, Gorelik J, Ostanin VP, Lab MJ, Moss GWJ, Frolenkov GI (co-corresponding author), Klenerman D, Korchev YE. (2009) Hopping probe ion conductance microscopy allows nanoscale imaging of live complex cellular structures. Nat. Methods 6: 279-281 (featured on the cover).
Stepanyan R, Frolenkov GI. (2009) Fast adaptation and Ca2+-sensitivity of the mechanotransducer require myosin-XVa in inner but not outer cochlear hair cells. J. Neurosci. 29: 4023-4034.