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Lab: (859) 323-7034
Ph.D. Emory University, 1989
Cardiac contractile function is reciprocally linked to cardiac morphogenesis during development. Therefore, to understand diseases of development we need to understand the basis for development of heart function. A second important reason to study heart development is that many embryonic genes are re-expressed in adult diseased states. Our lab studies the electrophysiological function of the heart during embryonic development, and adult diseased states. Our emphasis is on ion channel contributions to embryonic development and heart disease. Ion channels are integral membrane proteins that allow intracellular flux of ions from outside to inside cells. Ion channels are controlled by voltage and by a variety of intracellular kinases and phosphatases. A major focus of the lab is to study a novel mechanism whereby a proteolytic fragment of the L-type calcium channel serves a dual role - as a ionic current modulator, and as a transcription factor. A second major area of study is human heart development using human embryonic stem cells as a model system. A major benefit of study embryonic stem cell derived heart cells is their potential as a cell source for future cell replacement therapies for heart disease.
- Levitan, BM ; Ahern, BM ; Aloysius, A ; Brown, L.; Wen, Y.; Andres, DA ; Satin, J. "Rad-GTPase contributes to heart rate via L-type calcium channel regulation." Journal of molecular and cellular cardiology 154, (2021): 60-69. [PubMed Link] | [ Full text ]
- Miller, JM ; Meki, MH ; Ou, Q.; George, SA ; Gams, A ; Abouleisa, RRE ; Tang, XL ; Ahern, BM ; Giridharan, GA ; El-Baz, A.; Hill, BG ; Satin, J.; Conklin, DJ ; Moslehi, J ; Bolli, R.; Ribeiro, AJS ; Efimov, IR ; Mohamed, TMA "Heart slice culture system reliably demonstrates clinical drug-related cardiotoxicity." Toxicology and applied pharmacology 406, (2020): 115213. [PubMed Link] | [ Full text ]
- Andres, DA ; Young, LEA ; Veeranki, S.; Hawkinson, TR ; Levitan, BM ; He, D.; Wang, C.; Satin, J.; Sun, RC "Improved workflow for mass spectrometry-based metabolomics analysis of the heart." The Journal of biological chemistry 295, 9 (2020): 2676-2686. [PubMed Link] | [ Full text ]
- Dungan, CM ; Murach, KA ; Frick, KK ; , ; Crow, SE ; Englund, DA ; Vechetti IJ, Jr ; Figueiredo, VC ; Levitan, BM ; Satin, J.; McCarthy, JJ ; Peterson, CA "Elevated myonuclear density during skeletal muscle hypertrophy in response to training is reversed during detraining." American journal of physiology. Cell physiology 316, 5 (2019): C649-C654. [PubMed Link] | [ Full text ]
- Ahern, BM ; Levitan, BM ; Veeranki, S.; Shah, M.; Ali, N.; Sebastian, A.; Su, W.; Gong, MC ; Li, J.; Stelzer, JE ; Andres, DA ; Satin, J. "Myocardial-restricted ablation of the GTPase RAD results in a pro-adaptive heart response in mice." The Journal of biological chemistry 294, 28 (2019): 10913-10927. [PubMed Link] | [ Full text ]
- Ahern, BM ; Satin, J. "The L-type calcium channel current modulation mechanism: the plot thickens and fogs." The Journal of clinical investigation 129, 2 (2019): 496-498. [PubMed Link] |
- Manning, JR ; Chelvarajan, L.; Levitan, BM ; Withers, CN ; Nagareddy, PR ; Haggerty, CM ; Fornwalt, BK ; Gao, E.; Tripathi, H.; Abdel-Latif, A.; Andres, DA ; Satin, J. "Rad GTPase deletion attenuates post-ischemic cardiac dysfunction and remodeling." JACC. Basic to translational science 3, 1 (2018): 83-96. [PubMed Link] | [ Full text ]
- Withers, CN ; Brown, DM ; Byiringiro, I.; Allen, MR ; Condon, KW ; Satin, J.; Andres, DA "Rad GTPase is essential for the regulation of bone density and bone marrow adipose tissue in mice." Bone 103, (2017): 270-280. [PubMed Link] |
- Levitan, BM ; Manning, JR ; Withers, CN ; Smith, JD ; Shaw, RM ; Andres, DA ; Sorrell, VL ; Satin, J. "Rad-deletion Phenocopies Tonic Sympathetic Stimulation of the Heart." Journal of cardiovascular translational research 9, 5-6 (2016): 432-444. [PubMed Link] | [ Full text ]
- Manning, JR ; Withers, CN ; Levitan, B.; Smith, JD ; Andres, DA ; Satin, J. "Loss of Rad-GTPase produces a novel adaptive cardiac phenotype resistant to systolic decline with aging." American journal of physiology. Heart and circulatory physiology 309, 8 (2015): H1336-45. [PubMed Link] | [ Full text ]
- Magyar, J.; Bányász, T.; Szentandrássy, N.; Kistamás, K.; Nánási, PP ; Satin, J. "Role of gap junction channel in the development of beat-to-beat action potential repolarization variability and arrhythmias." Current pharmaceutical design 21, 8 (2015): 1042-52. [PubMed Link] |
- Yin, G.; Hassan, F.; Haroun, AR ; Murphy, LL ; Crotti, L.; Schwartz, PJ ; George, AL ; Satin, J. "Arrhythmogenic calmodulin mutations disrupt intracellular cardiomyocyte Ca2+ regulation by distinct mechanisms." Journal of the American Heart Association 3, 3 (2014): e000996. [PubMed Link] | [ Full text ]
- Crump, SM ; Andres, DA ; Sievert, G.; Satin, J. "The cardiac L-type calcium channel distal carboxy terminus autoinhibition is regulated by calcium." American journal of physiology. Heart and circulatory physiology 304, 3 (2013): H455-64. [PubMed Link] | [ Full text ]
- Manning, JR ; Yin, G.; Kaminski, CN ; Magyar, J.; Feng, HZ ; Penn, J.; Sievert, G.; Thompson, K.; Jin, JP ; Andres, DA ; Satin, J. "Rad GTPase deletion increases L-type calcium channel current leading to increased cardiac contraction." Journal of the American Heart Association 2, 6 (2013): e000459. [PubMed Link] |
- Kiper, C.; Grimes, B.; Van Zant, G.; Satin, J. "Mouse strain determines cardiac growth potential." PloS one 8, 8 (2013): e70512. [PubMed Link] | [ Full text ]
- Gunton, JE ; Sisavanh, M.; Stokes, RA ; Satin, J.; Satin, LS ; Zhang, M.; Liu, SM ; Cai, W.; Cheng, K.; Cooney, GJ ; Laybutt, DR ; So, T.; Molero, JC ; Grey, ST ; Andres, DA ; Rolph, MS ; Mackay, CR "Mice deficient in GEM GTPase show abnormal glucose homeostasis due to defects in beta-cell calcium handling." PloS one 7, 6 (2012): e39462. [PubMed Link] | [ Full text ]
- Satin, J.; Schroder, EA ; Crump, SM "L-type calcium channel auto-regulation of transcription." Cell calcium 49, 5 (2011): 306-13. [PubMed Link] | [ Full text ]
- Magyar, J.; Jenes, A.; Kistamás, K.; Ruzsnavszky, F.; Nánási, PP ; Satin, J.; Szentandrássy, N.; Bányász, T. "Long term regulation of cardiac L-type calcium channel by small G proteins." Current medicinal chemistry 18, 24 (2011): 3714-9. [PubMed Link] | [ Full text ]
- Satin, J.; Schroder, EA "Autoregulation of cardiac l-type calcium channels." Trends in cardiovascular medicine 19, 8 (2009): 268-71. [PubMed Link] | [ Full text ]
- Schroder, E.; Byse, M.; Satin, J. "L-type calcium channel C terminus autoregulates transcription." Circulation research 104, 12 (2009): 1373-81. [PubMed Link] | [ Full text ]
- Lester, WC ; Schroder, EA ; Burgess, DE ; Yozwiak, D.; Andres, DA ; Satin, J. "Steady-state coupling of plasma membrane calcium entry to extrusion revealed by novel L-type calcium channel block." Cell calcium 44, 4 (2008): 353-62. [PubMed Link] | [ Full text ]
- Correll, RN ; Pang, C.; Niedowicz, DM ; Satin, J.; Andres, DA "Calmodulin binding is dispensable for Rem-mediated Ca2+ channel inhibition." Molecular and cellular biochemistry 310, 1-2 (2008): 103-10. [PubMed Link] | [ Full text ]
- Correll, RN ; Botzet, GJ ; Satin, J.; Andres, DA ; Finlin, BS "Analysis of the Rem2 - voltage dependant calcium channel beta subunit interaction and Rem2 interaction with phosphorylated phosphatidylinositide lipids." Cellular signalling 20, 2 (2008): 400-8. [PubMed Link] |
- Satin, J.; Itzhaki, I.; Rapoport, S.; Schroder, EA ; Izu, L.; Arbel, G.; Beyar, R.; Balke, CW ; Schiller, J.; Gepstein, L. "Calcium handling in human embryonic stem cell-derived cardiomyocytes." Stem cells (Dayton, Ohio) 26, 8 (2008): 1961-72. [PubMed Link] | [ Full text ]
- Correll, RN ; Pang, C.; Finlin, BS ; Dailey, AM ; Satin, J.; Andres, DA "Plasma membrane targeting is essential for Rem-mediated Ca2+ channel inhibition." The Journal of biological chemistry 282, 39 (2007): 28431-28440. [PubMed Link] | [ Full text ]
- Schroder, E.; Magyar, J.; Burgess, D.; Andres, D.; Satin, J. "Chronic verapamil treatment remodels ICa,L in mouse ventricle." American journal of physiology. Heart and circulatory physiology 292, 4 (2007): H1906-16. [PubMed Link] | [ Full text ]
- Itzhaki, I.; Schiller, J.; Beyar, R.; Satin, J.; Gepstein, L. "Calcium handling in embryonic stem cell-derived cardiac myocytes: of mice and men." Annals of the New York Academy of Sciences 1080, (2006): 207-15. [PubMed Link] | [ Full text ]
- Schroder, EA ; Wei, Y.; Satin, J. "The developing cardiac myocyte: maturation of excitability and excitation-contraction coupling." Annals of the New York Academy of Sciences 1080, (2006): 63-75. [PubMed Link] | [ Full text ]
- Crump, SM ; Correll, RN ; Schroder, EA ; Lester, WC ; Finlin, BS ; Andres, DA ; Satin, J. "L-type calcium channel alpha-subunit and protein kinase inhibitors modulate Rem-mediated regulation of current." American journal of physiology. Heart and circulatory physiology 291, 4 (2006): H1959-71. [PubMed Link] | [ Full text ]
- Finlin, BS ; Correll, RN ; Pang, C.; Crump, SM ; Satin, J.; Andres, DA "Analysis of the complex between Ca2+ channel beta-subunit and the Rem GTPase." The Journal of biological chemistry 281, 33 (2006): 23557-66. [PubMed Link] | [ Full text ]
- Andres, DA ; Crump, SM ; Correll, RN ; Satin, J.; Finlin, BS "Analyses of Rem/RGK signaling and biological activity." Methods in enzymology 407, (2006): 484-98. [PubMed Link] | [ Full text ]
- Finlin, BS ; Mosley, AL ; Crump, SM ; Correll, RN ; Ozcan, S.; Satin, J.; Andres, DA "Regulation of L-type Ca2+ channel activity and insulin secretion by the Rem2 GTPase." The Journal of biological chemistry 280, 51 (2005): 41864-71. [PubMed Link] | [ Full text ]
- Satin, J.; Kehat, I.; Caspi, O.; Huber, I.; Arbel, G.; Itzhaki, I.; Magyar, J.; Schroder, EA ; Perlman, I.; Gepstein, L. "Mechanism of spontaneous excitability in human embryonic stem cell derived cardiomyocytes." The Journal of physiology 559, Pt 2 (2004): 479-96. [PubMed Link] | [ Full text ]
- Kehat, I.; Khimovich, L.; Caspi, O.; Gepstein, A.; Shofti, R.; Arbel, G.; Huber, I.; Satin, J.; Itskovitz-Eldor, J.; Gepstein, L. "Electromechanical integration of cardiomyocytes derived from human embryonic stem cells." Nature biotechnology 22, 10 (2004): 1282-9. [PubMed Link] |
- Magyar, J.; Kiper, CE ; Dumaine, R.; Burgess, DE ; Bányász, T.; Satin, J. "Divergent action potential morphologies reveal nonequilibrium properties of human cardiac Na channels." Cardiovascular research 64, 3 (2004): 477-87. [PubMed Link] | [ Full text ]
- Finlin, BS ; Crump, SM ; Satin, J.; Andres, DA "Regulation of voltage-gated calcium channel activity by the Rem and Rad GTPases." Proceedings of the National Academy of Sciences of the United States of America 100, 24 (2003): 14469-74. [PubMed Link] |
- Delisle, BP ; Satin, J. "Monovalent cations contribute to T-type calcium channel (Cav3.1 and Cav3.2) selectivity." The Journal of membrane biology 193, 3 (2003): 185-94. [PubMed Link] | [ Full text ]
- Burgess, DE ; Crawford, O.; Delisle, BP ; Satin, J. "Mechanism of inactivation gating of human T-type (low-voltage activated) calcium channels." Biophysical journal 82, 4 (2002): 1894-906. [PubMed Link] | [ Full text ]
- Cribbs, LL ; Martin, BL ; Schroder, EA ; Keller, BB ; Delisle, BP ; Satin, J. "Identification of the t-type calcium channel (Ca(v)3.1d) in developing mouse heart." Circulation research 88, 4 (2001): 403-7. [PubMed Link] |
- Satin, J.; Cribbs, LL "Identification of a T-type Ca(2+) channel isoform in murine atrial myocytes (AT-1 cells)" Circulation research 86, 6 (2000): 636-42. [PubMed Link] |
- Zhang, Y.; Cribbs, LL ; Satin, J. "Arachidonic acid modulation of alpha1H, a cloned human T-type calcium channel." American journal of physiology. Heart and circulatory physiology 278, 1 (2000): H184-93. [PubMed Link] | [ Full text ]
- Delisle, BP ; Satin, J. "pH modification of human T-type calcium channel gating." Biophysical journal 78, 4 (2000): 1895-905. [PubMed Link] | [ Full text ]
- Zhang, Y.; Hartmann, HA ; Satin, J. "Glycosylation influences voltage-dependent gating of cardiac and skeletal muscle sodium channels." The Journal of membrane biology 171, 3 (1999): 195-207. [PubMed Link] | [ Full text ]
- Cribbs, LL ; Lee, JH ; Yang, J.; Satin, J.; Zhang, Y.; Daud, A.; Barclay, J.; Williamson, MP ; Fox, M.; Rees, M.; Perez-Reyes, E. "Cloning and characterization of alpha1H from human heart, a member of the T-type Ca2+ channel gene family." Circulation research 83, 1 (1998): 103-9. [PubMed Link] | [ Full text ]
- Chang, SY ; Satin, J.; Fozzard, HA "Modal behavior of the mu 1 Na+ channel and effects of coexpression of the beta 1-subunit." Biophysical journal 70, 6 (1996): 2581-92. [PubMed Link] | [ Full text ]
- Satin, J.; Limberis, JT ; Kyle, JW ; Rogart, RB ; Fozzard, HA "The saxitoxin/tetrodotoxin binding site on cloned rat brain IIa Na channels is in the transmembrane electric field." Biophysical journal 67, 3 (1994): 1007-14. [PubMed Link] | [ Full text ]
- Satin, J.; Kyle, JW ; Fan, Z.; Rogart, R.; Fozzard, HA ; Makielski, JC "Post-repolarization block of cloned sodium channels by saxitoxin: the contribution of pore-region amino acids." Biophysical journal 66, 5 (1994): 1353-63. [PubMed Link] | [ Full text ]
- Doyle, DD ; Guo, Y.; Lustig, SL ; Satin, J.; Rogart, RB ; Fozzard, HA "Divalent cation competition with 3H saxitoxin binding to tetrodotoxin-resistant and -sensitive sodium channels. A two-site structural model of ion/toxin interaction." The Journal of general physiology 101, 2 (1993): 153-82. [PubMed Link] | [ Full text ]
- Makielski, JC ; Satin, J.; Fan, Z. "Post-repolarization block of cardiac sodium channels by saxitoxin." Biophysical journal 65, 2 (1993): 790-8. [PubMed Link] |
- Satin, J.; Kyle, JW ; Chen, M.; Rogart, RB ; Fozzard, HA "The cloned cardiac Na channel alpha-subunit expressed in Xenopus oocytes show gating and blocking properties of native channels." The Journal of membrane biology 130, 1 (1992): 11-22. [PubMed Link] |
- Satin, J.; Kyle, JW ; Chen, M.; Bell, P.; Cribbs, LL ; Fozzard, HA ; Rogart, RB "A mutant of TTX-resistant cardiac sodium channels with TTX-sensitive properties." Science (New York, N.Y.) 256, 5060 (1992): 1202-5. [PubMed Link] | [ Full text ]
- Cribbs, LL ; Satin, J.; Fozzard, HA ; Rogart, RB "Functional expression of the rat heart I Na+ channel isoform. Demonstration of properties characteristic of native cardiac Na+ channels." FEBS letters 275, 1-2 (1990): 195-200. [PubMed Link] | [ Full text ]
- Satin, J.; Fujii, S.; DeHaan, RL "Development of cardiac beat rate in early chick embryos is regulated by regional cues." Developmental biology 129, 1 (1988): 103-13. [PubMed Link] | [ Full text ]